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You searched for: subject:"genomics"
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[texts]Genomics via Optical Mapping (I): 0-1 Laws for Mapping with Single Molecules - Thomas Anantharaman
The genomic data that can be collected from a single DNA molecule by the best chemical and optical methods (e.g., using technologies from OpGen, BioNanoGenomics, NABSys, PacBio, etc.) are badly corrupted by many poorly understood noise processes. Thus, single molecule technology derives its utility through powerful probabilistic modeling, which can provide precise lower and upper bounds on various experimental parameters to create the correct map or validate sequence assembly...
Keywords: Genomics
[texts]Illumina TruSeq synthetic long-reads empower de novo assembly and resolve complex, highly repetitive transposable elements - Rajiv C McCoy
High-throughput DNA sequencing technologies have revolutionized genomic analysis, including the de novo assembly of whole genomes. Nevertheless, assembly of complex genomes remains challenging, in part due to the presence of dispersed repeats which introduce ambiguity during genome reconstruction. Transposable elements (TEs) can be particularly problematic, especially for TE families exhibiting high sequence identity, high copy number, or present in complex genomic arrangements...
Keywords: Genomics
[texts]A genome wide dosage suppressor network reveals genetic robustness and a novel mechanism for Huntington’s disease - Biranchi Patra
Mutational robustness is the extent to which an organism has evolved to withstand the effects of deleterious mutations. We explored the extent of mutational robustness in the budding yeast by genome wide dosage suppressor analysis of 53 conditional lethal mutations in cell division cycle and RNA synthesis related genes, revealing 660 suppressor interactions of which 642 are novel. This collection has several distinctive features, including high co-occurrence of mutant-suppressor pairs within pro...
Keywords: Genomics
[texts]Avoiding test set bias with rank-based prediction - Prasad Patil
Background: Prior to applying genomic predictors to clinical samples, the genomic data must be properly normalized. The most effective normalization methods depend on the data from multiple patients. From a biomedical perspective this implies that predictions for a single patient may change depending on which other patient samples they are normalized with. This test set bias will occur when any cross-sample normalization is used before clinical prediction...
Keywords: Genomics
[texts]Copy number networks to guide combinatorial therapy for cancer and other disorders - Andy Lin
The dwindling drug pipeline is driving increased interest in the use of genome datasets to inform drug treatment. In particular, networks based on transcript data and protein-protein interactions have been used to design therapies that employ drug combinations. But there has been less focus on employing human genetic interaction networks constructed from copy number alterations (CNAs). These networks can be charted with sensitivity and precision by seeking gene pairs that tend to be amplified an...
Keywords: Genomics
[texts]Preparation of next-generation DNA sequencing libraries from ultra-low amounts of input DNA: Application to single-molecule, real-time (SMRT) sequencing on the Pacific Biosciences RS II. - Castle Raley
We have developed and validated an amplification-free method for generating DNA sequencing libraries from very low amounts of input DNA (500 picograms - 20 nanograms) for single-molecule sequencing on the Pacific Biosciences (PacBio) RS II sequencer. The common challenge of high input requirements for single-molecule sequencing is overcome by using a carrier DNA in conjunction with optimized sequencing preparation conditions and re-use of the MagBead-bound complex...
Keywords: Genomics
[texts]Quantitative comparison of single-cell sequencing methods using hippocampal neurons - Luwen Ning
Single-cell genomic analysis has grown rapidly in recent years and will find widespread applications in various fields of biology, including cancer biology, development, immunology, pre-implantation genetic diagnosis, and neurobiology. In this study, we amplified genomic DNA from individual hippocampal neurons using one of three single-cell DNA amplification methods (multiple annealing and looping-based amplification cycles (MALBAC), multiple displacement amplification (MDA), and GenomePlex whol...
Keywords: Genomics
[texts]Epigenetic Modifications are Associated with Inter-species Gene Expression Variation in Primates - Xiang Zhou
Changes in gene regulation level have long been thought to play an important role in evolution and speciation, especially in primates. Over the past decade, comparative genomic studies have revealed extensive inter-species differences in gene expression levels yet we know much less about the extent to which regulatory mechanisms differ between species. To begin addressing this gap, we performed a comparative epigenetic study in primate lymphoblastoid cell lines (LCLs), to query the contribution ...
Keywords: Genomics
[texts]Genome sequencing of the perciform fish Larimichthys crocea provides insights into stress adaptation - Jingqun Ao
The large yellow croaker Larimichthys crocea (L. crocea) is one of the most economically important marine fish in China and East Asian countries. It also exhibits peculiar behavioral and physiological characteristics, especially sensitive to various environmental stresses, such as hypoxia and air exposure. These traits may render L. crocea a good model for investigating the response mechanisms to environmental stress...
Keywords: Genomics
[texts]Genome-wide Comparative Analysis Reveals Possible Common Ancestors of NBS Domain Containing Genes in Hybrid Citrus sinensis Genome and Original Citrus clementina Genome - Yunsheng Wang
Background Recently available whole genome sequences of three citrus species: one Citrus clementina and two Citrus sinensis genomes have made it possible to understand the features of candidate disease resistance genes with nucleotide-binding sites (NBS) domain in Citrus and how NBS genes differ between hybrid and original Citrus species. Result We identified and re-annotated NBS genes from three citrus genomes and found similar numbers of NBS genes in those citrus genomes...
Keywords: Genomics
[texts]Population split time estimation and X to autosome effective population size differences inferred using physically phased genomes - Shiya Song
Haplotype resolved genome sequence information is of growing interest due to its applications in both population genetics and medical genetics. Here, we assess the ability to correctly reconstruct haplotype sequences using fosmid pooled sequencing and apply the sequences to explore historical population relationships. We resolved phased haplotypes of sample NA19240, a trio child from the Yoruba HapMap collection using pools of a total of 521,783 fosmid clones...
Keywords: Genomics
[texts]Highly epistatic genetic architecture of root length in Arabidopsis thaliana - Jennifer Lachowiec
Efforts to identify loci underlying complex traits generally assume that most genetic variance is additive. This is despite the fact that non-additive genetic effects, such as epistatic interactions and developmental noise, are also likely to make important contributions to the phenotypic variability. Analyses beyond additivity require additional care in the design and collection of data, and introduce significant analytical and computational challenges in the statistical analyses...
Keywords: Genomics
[texts]Are all genetic variants in DNase I sensitivity regions functional? - Gregory A Moyerbrailean
A detailed mechanistic understanding of the direct functional consequences of DNA variation on gene regulatory mechanism is critical for a complete understanding of complex trait genetics and evolution. Here, we present a novel approach that integrates sequence information and DNase I footprinting data to predict the impact of a sequence change on transcription factor binding. Applying this approach to 653 DNase-seq samples, we identified 3,831,862 regulatory variants predicted to affect active ...
Keywords: Genomics
[texts]The genomic landscape of polymorphic human nuclear mitochondrial insertions - Gargi Dayama
The transfer of mitochondrial genetic material into the nuclear genomes of eukaryotes is a well-established phenomenon. Many studies over the past decade have utilized reference genome sequences of numerous species to characterize the prevalence and contribution of nuclear mitochondrial insertions to human diseases. The recent advancement of high throughput sequencing technologies has enabled the interrogation of genomic variation at a much finer scale, and now allows for an exploration into the...
Keywords: Genomics
[texts]Facile semi-automated forensic body fluid identification by multiplex solution hybridization of NanoString® barcode probes to specific mRNA targets - Patrick Danaher
A DNA profile from the perpetrator does not reveal, per se, the circumstances by which it was transferred. Body fluid identification by mRNA profiling may allow extraction of contextual 'activity level' information from forensic samples. Here we describe the development of a prototype multiplex digital gene expression (DGE) method for forensic body fluid/tissue identification based upon solution hybridization of color-coded NanoString® probes to 23 tissue/body fluid specific mRNA targets...
Keywords: Genomics
[texts]Methylation QTLs are associated with coordinated changes in transcription factor binding, histone modifications, and gene expression levels. - Nicholas E Banovich
DNA methylation is an important epigenetic regulator of gene expression. Recent studies have revealed widespread associations between genetic variation and methylation levels. However, the mechanistic links between genetic variation and methylation remain unclear. To begin addressing this gap, we collected methylation data at ~300,000 loci in lymphoblastoid cell lines (LCLs) from 64 HapMap Yoruba individuals, and genome-wide bisulfite sequence data in ten of these individuals...
Keywords: Genomics
[texts]Single haplotype assembly of the human genome from a hydatidiform mole - Karyn Meltz Steinberg
An accurate and complete reference human genome sequence assembly is essential for accurately interpreting individual genomes and associating sequence variation with disease phenotypes. While the current reference genome sequence is of very high quality, gaps and misassemblies remain due to biological and technical complexities. Large repetitive sequences and complex allelic diversity are the two main drivers of assembly error...
Keywords: Genomics
[texts]Ligation-anchored PCR unveils immune repertoire of TCR-beta from whole blood - Fan Gao
Background As one of the genetic mechanisms for adaptive immunity, V(D)J recombination generates an enormous repertoire of T-cell receptors (TCRs). With the development of high-throughput sequencing techniques, systematic exploration of V(D)J recombination becomes possible. Multiplex PCR method has been previously developed to assay immune repertoire, however the usage of primer pools has inherent bias in target amplification...
Keywords: Genomics
[texts]Probabilities of Fitness Consequences for Point Mutations Across the Human Genome - Brad Gulko
We describe a novel computational method for estimating the probability that a point mutation at each position in a genome will influence fitness. These fitness consequence (fitCons) scores serve as evolution-based measures of potential genomic function. Our approach is to cluster genomic positions into groups exhibiting distinct "fingerprints" based on high-throughput functional genomic data, then to estimate a probability of fitness consequences for each group from associated patterns of genet...
Keywords: Genomics
[texts]Improved genome inference in the MHC using a population reference graph - Alexander Dilthey
In humans and many other species, while much is known about the extent and structure of genetic variation, such information is typically not used in assembling novel genomes. Rather, a single reference is used against which to map reads, which can lead to poor characterisation of regions of high sequence or structural diversity. Here, we introduce a population reference graph, which combines multiple reference sequences as well as catalogues of SNPs and short indels...
Keywords: Genomics
[texts]Long non-coding RNA discovery in Anopheles gambiae using deep RNA sequencing - Adam M Jenkins
Long non-coding RNAs (lncRNAs) are mRNA-like transcripts longer than 200 bp that have no protein-coding potential. lncRNAs have recently been implicated in epigenetic regulation, transcriptional and post-transcriptional gene regulation, and regulation of genomic stability in mammals, Caenorhabditis elegans, and Drosophila melanogaster. Using deep RNA sequencing of multiple Anopheles gambiae life stages, we have identified over 600 novel lncRNAs and more than 200 previously unannotated putative p...
Keywords: Genomics
[texts]Genome-wide Identification of Zero Nucleotide Recursive Splicing in Drosophila - Michael O Duff
Recursive splicing is a process in which large introns are removed in multiple steps by resplicing at ratchet points - 5? splice sites recreated after splicing. Recursive splicing was first identified in the Drosophila Ultrabithorax (Ubx) gene and only three additional Drosophila genes have since been experimentally shown to undergo recursive splicing. Here, we identify 196 zero nucleotide exon ratchet points in 130 introns of 115 Drosophila genes from total RNA sequencing data generated from de...
Keywords: Genomics
[texts]Autosomal admixture levels are informative about sex bias in admixed populations - Amy Goldberg
Sex-biased admixture has been observed in a wide variety of admixed populations. Genetic variation in sex chromosomes and ratios of quantities computed from sex chromosomes and autosomes have often been examined in order to infer patterns of sex-biased admixture, typically using statistical approaches that do not mechanistically model the complexity of a sex-specific history of admixture. Here, expanding on a model of Verdu \& Rosenberg (2011) that did not include sex specificity, we develop a m...
Keywords: Genomics
[texts]Estimating cellular pathways from an ensemble of heterogeneous data sources - Alexander Franks
Building better models of cellular pathways is one of the major challenges of systems biology and functional genomics. There is a need for methods to build on established expert knowledge and reconcile it with results of high-throughput studies. Moreover, the available data sources are heterogeneous and need to be combined in a way specific for the part of the pathway in which they are most informative...
Keywords: Genomics
[texts]Redefining Genomic Privacy: Trust and Empowerment - Arvind Narayanan
Fulfilling the promise of the genetic revolution requires the analysis of large datasets containing information from thousands to millions of participants. However, sharing human genomic data requires protecting subjects from potential harm. Current models rely on de-identification techniques that treat privacy versus data utility as a zero-sum game. Instead we propose using trust-enabling techniques to create a solution where researchers and participants both win...
Keywords: Genomics
[texts]Complete plastid genome assembly of invasive plant, Centaurea diffusa - Kathryn G Turner
New genomic tools are needed to elucidate the evolution of invasive, non-model organisms. Here we present the completed plastome assembly for the problematic invasive weed, Centaurea diffusa. This new tool represents a significant contribution to future studies of the ecological genomics of invasive plants, particularly this weedy genus, and studies of the Asteraceae in general.
Keywords: Genomics
[texts]Alternative splicing detection workflow needs a careful combination of sample prep and bioinformatics analysis - Matteo Carrara
Background RNAseq provides remarkable power in the area of biomarkers discovery and disease stratification. The main technical steps affecting the results of RNAseq experiments are Library Sample Preparation (LSP) and Bioinformatics Analysis (BA). At the best of our knowledge, a comparative evaluation of the combined effect of LSP and BA was never considered and it might represent a valuable knowledge to optimize alternative splicing detection, which is a challenging task due to moderate fold ch...
Keywords: Genomics
[texts]CasFinder: Flexible algorithm for identifying specific Cas9 targets in genomes - John Aach
CRISPR/Cas9 systems enable many molecular activities to be efficiently directed in vivo to user-specifiable DNA sequences of interest, including generation of dsDNA cuts and nicks, transcriptional activation and repression, and fluorescence. CRISPR targeting relies on base pairing of short RNA transcripts with their target DNA sequences that must also be adjacent to fixed DNA motifs. However, rules for Cas9 targeting specificity are incompletely known...
Keywords: Genomics
[texts]Power analysis of artificial selection experiments using efficient whole genome simulation of quantitative traits - Darren Kessner
Evolve and resequence studies combine artificial selection experiments with massively parallel sequencing technology to study the genetic basis for complex traits. In these experiments, individuals are selected for extreme values of a trait, causing alleles at quantitative trait loci (QTLs) to increase or decrease in frequency in the experimental population. We present a new analysis of the power of artificial selection experiments to detect and localize quantitative trait loci...
Keywords: Genomics
[texts]Nuclear stability and transcriptional directionality separate functionally distinct RNA species - Robin Andersson
Mammalian genomes are pervasively transcribed, yielding a complex transcriptome with high variability in composition and cellular abundance. While recent efforts have identified thousands of new long non-coding (lnc) RNAs and demonstrated a complex transcriptional repertoire produced by protein-coding (pc) genes, limited progress has been made in distinguishing functional RNA from spurious transcription events...
Keywords: Genomics
[texts]Phylogenetic Identification and Functional Characterization of Orthologs and Paralogs across Human, Mouse, Fly, and Worm - Yi-Chieh Wu
Model organisms can serve the biological and medical community by enabling the study of conserved gene families and pathways in experimentally-tractable systems. Their use, however, hinges on the ability to reliably identify evolutionary orthologs and paralogs with high accuracy, which can be a great challenge at both small and large evolutionary distances. Here, we present a phylogenomics-based approach for the identification of orthologous and paralogous genes in human, mouse, fly, and worm, w...
Keywords: Genomics
[texts]Profiling direct mRNA-microRNA interactions using synthetic biotinylated microRNA-duplexes - Shivangi Wani
MicroRNAs (miRNAs) are predominantly negative regulators of gene expression that act through the RNA-induced Silencing Complex (RISC) to suppress the translation of protein coding mRNAs. Despite intense study of these regulatory molecules, the specific molecular functions of most miRNAs remain unknown, largely due to the challenge of accurately identifying miRNA targets. Reporter gene assays can determine direct interactions, but are laborious and do not scale to genome-wide screens...
Keywords: Genomics
[texts]Human genomic regions with exceptionally high or low levels of population differentiation identified from 911 whole-genome sequences - Vincenza Colonna
Background: Population differentiation has proved to be effective for identifying loci under geographically-localized positive selection, and has the potential to identify loci subject to balancing selection. We have previously investigated the pattern of genetic differentiation among human populations at 36.8 million genomic variants to identify sites in the genome showing high frequency differences...
Keywords: Genomics
[texts]Characterizing a collective and dynamic component of chromatin immunoprecipitation enrichment profiles in yeast - Lucas D. Ward
Recent chromatin immunoprecipitation (ChIP) experiments in fly, mouse, and human have revealed the existence of high-occupancy target (HOT) regions or hotspots that show enrichment across many assayed DNA-binding proteins. Similar co-enrichment observed in yeast so far has been treated as artifactual, and has not been fully characterized. Here we reanalyze ChIP data from both array-based and sequencing-based experiments to show that in the yeast S...
Keywords: Genomics
[texts]Crowdsourced analysis of ash and ash dieback through the Open Ash Dieback project: A year 1 report on datasets and analyses contributed by a self-organising community. - Diane Saunders
Ash dieback is a fungal disease of ash trees caused by Hymenoscyphus pseudoalbidus that has swept across Europe in the last two decades and is a significant threat to the ash population. This emergent pathogen has been relatively poorly studied and little is known about its genetic make-up. In response to the arrival of this dangerous pathogen in the UK we took the unusual step of providing an open access database and initial sequence datasets to the scientific community for analysis prior to pe...
Keywords: Genomics
[texts]Diversity and evolution of centromere repeats in the maize genome - Paul Bilinski
Centromere repeats are found in most eukaryotes and play a critical role in kinetochore formation. Though CentC repeats exhibit considerable diversity both within and among species, little is understood about the mechanisms that drive cen- tromere repeat evolution. Here, we use maize as a model to investigate how a complex history involving polyploidy, fractionation, and recent domestication has impacted the diversity of the maize CentC repeat...
Keywords: Genomics
[texts]The Landscape of Human STR Variation - Thomas F. Willems
Short Tandem Repeats are among the most polymorphic loci in the human genome. These loci play a role in the etiology of a range of genetic diseases and have been frequently utilized in forensics, population genetics, and genetic genealogy. Despite this plethora of applications, little is known about the variation of most STRs in the human population. Here, we report the largest-scale analysis of human STR variation to date...
Keywords: Genomics
Downloads: 3
[texts]The causal meaning of genomic predictors and how it affects the construction and comparison of genome-enabled selection models - Bruno D Valente
The additive genetic effect is arguably the most important quantity inferred in animal and plant breeding analyses. The term effect indicates that it represents causal information, which is different from standard statistical concepts as regression coefficient and association. The process of inferring causal information is also different from standard statistical learning, as the former requires causal (i.e...
Keywords: Genomics
Downloads: 2
[texts]SRST2: Rapid genomic surveillance for public health and hospital microbiology labs - Michael Inouye
Rapid molecular typing of bacterial pathogens is critical for public health epidemiology, surveillance and infection control, yet routine use of whole genome sequencing (WGS) for these purposes poses significant challenges. Here we present SRST2, a read mapping-based tool for fast and accurate detection of genes, alleles and multi-locus sequence types (MLST) from WGS data. Using >900 genomes from common pathogens, we show SRST2 is highly accurate and outperforms assembly-based methods in terms o...
Keywords: Genomics
Downloads: 2
[texts]Genetics of single-cell protein abundance variation in large yeast populations - Frank Albert
Many DNA sequence variants influence phenotypes by altering gene expression. Our understanding of these variants is limited by sample sizes of current studies and by measurements of mRNA rather than protein abundance. We developed a powerful method for identifying genetic loci that influence protein expression in very large populations of the yeast Saccharomyes cerevisiae. The method measures single-cell protein abundance through the use of green-fluorescent-protein tags...
Keywords: Genomics
Downloads: 2
[texts]THE GENETIC LANDSCAPE OF TRANSCRIPTIONAL NETWORKS IN A COMBINED HAPLOID/DIPLOID PLANT SYSTEM - Jukka-Pekka Verta
Heritable variation in gene expression is a source of evolutionary change and our understanding of the genetic basis of expression variation remains incomplete. Here, we dissected the genetic basis of transcriptional variation in a wild, outbreeding gymnosperm (Picea glauca) according to linked and unlinked genetic variants, their allele-specific (cis) and allele non-specific (trans) effects, and their phenotypic additivity...
Keywords: Genomics
[texts]Accounting for biases in riboprofiling data indicates a major role for proline and not positive amino acids in stalling translation - Carlo G. Artieri
The recent advent of ribosome profiling ? sequencing of short ribosome-bound fragments of mRNA ? has offered an unprecedented opportunity to interrogate the sequence features responsible for modulating translational rates. Nevertheless, numerous analyses of the first riboprofiling dataset have produced equivocal and often incompatible results. Here we analyze three independent yeast riboprofiling data sets, including two with much higher coverage than previously available, and find that all thre...
Keywords: Genomics
Downloads: 2
[texts]Origins and impacts of new exons - Jason Merkin*
Mammalian genes are typically broken into several protein-coding and non-coding exons, but the evolutionary origins and functions of new exons are not well understood. Here, we analyzed patterns of exon gain using deep cDNA sequencing data from several mammals and one bird, identifying thousands of species- and lineage-specific exons. While exons conserved across mammals are mostly protein-coding and constitutively spliced, species-specific exons were mostly located in 5' untranslated regions an...
Keywords: Genomics
Downloads: 2
[texts]Modeling the functional relationship network at the splice isoform level through heterogeneous data integration - Hongdong Li
Functional relationship networks, which reveal the collaborative roles between genes, have significantly accelerated our understanding of gene functions and phenotypic relevance. However, establishing such networks for alternatively spliced isoforms remains a difficult, unaddressed problem due to the lack of systematic functional annotations at the isoform level, which renders most supervised learning methods difficult to be applied to isoforms...
Keywords: Genomics
Downloads: 2
[texts]mInDel: an efficient pipeline for high-throughput InDel marker discovery - Yuanda Lv
Next-Generation Sequencing (NGS) technologies have emerged as a powerful tool to reveal nucleotide polymorphisms in a high-throughput and cost-effective manner. However, it remains a daunting task to proficiently analyze the enormous volume of data generated from NGS and to identify length polymorphisms for molecular marker discovery. The development of insertion-deletion polymorphism (InDel) markers is in particular computationally intensive, calling for integrated high performance methods to i...
Keywords: Genomics
Downloads: 1
[texts]Joint annotation of chromatin state and chromatin conformation reveals relationships among domain types and identifies domains of cell type-specific expression - Maxwell W Libbrecht
The genomic neighborhood of a gene influences its activity, a behavior that is attributable in part to domain-scale regulation, in which regions of hundreds or thousands of kilobases known as domains are regulated as a unit. Previous studies using genomics assays such as chromatin immunoprecipitation (ChIP)-seq and chromatin conformation capture (3C)-based assays have identified many types of regulatory domains...
Keywords: Genomics
Downloads: 4
[texts]Distinct structural transitions of chromatin topological domains coordinate hormone-induced gene regulation - François Le Dily
The human genome is segmented into Topologically Associating Domains (TADs), but the role of this conserved organization during transient changes in gene expression is not known. Here we described the distribution of Progestin-induced chromatin modifications and changes in transcriptional activity over TADs in T47D breast cancer cells. Using ChIP-Seq, Hi-C and 3D modelling techniques, we found that the borders of the ~2,000 TADs in these cells are largely maintained after hormone treatment but t...
Keywords: Genomics
Downloads: 5
[texts]An introduction to LifeLines DEEP: study design and baseline characteristics - Ettje F Tigchelaar
There is a critical need for population-based prospective cohort studies because they follow individuals before the onset of disease, allowing for studies that can identify biomarkers and disease-modifying effects and thereby contributing to systems epidemiology. This paper describes the design and baseline characteristics of an intensively examined subpopulation of the LifeLines cohort in the Netherlands...
Keywords: Genomics
Downloads: 1
[texts]Transcriptome pyrosequencing of abnormal phenotypes in Trypanosoma cruzi epimastigotes after ectopic expression of a small zinc finger protein - Gaston Westergaard
The TcZFPs are a family of small zinc finger proteins harboring WW domains or Proline rich motifs. In Trypanosoma brucei, ZFPs are involved during stage specific differentiation. TcZFPs interact with each other using the WW domain (ZFP2 and ZFP3) and the proline rich motif (ZFP1). The tcZFP1b member is exclusive to Trypanosoma cruzi and it is only expressed in trypomastigote stage. We used a tetracycline inducible vector to express ectopically tcZFP1b in the epimastigote stage...
Keywords: Genomics
Downloads: 2
[texts]Assessing the prevalence of mycoplasma contamination in cell culture via a survey of NCBI?s RNA-seq archive - Anthony O Olarerin-George
Mycoplasmas are notorious contaminants of cell culture and can have profound effects on host cell biology by depriving cells of nutrients and inducing global changes in gene expression. Because they are small, they can escape filtration in culture media. Because they lack cell walls, they are resistant to commonly used antibiotics. Over the last two decades, sentinel testing has revealed wide-ranging contamination rates in mammalian culture...
Keywords: Genomics
Downloads: 14
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